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recombinant mouse vcam1 fc  (R&D Systems)


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    R&D Systems recombinant mouse vcam1 fc
    (A) Schematic of the workflow used to assess the effect of constitutive CRISPR/Cas9-mediated KO of <t>Vcam1</t> or Gpr37l1 on hippocampal synapse development. SO: stratum oriens, SR: stratum radiatum, SLM: stratum lacunosum-moleculare, SL: stratum lucidum, DG: dentate gyrus, ML: molecular layer. Created with BioRender.com. (B) Quality control of Vcam1 KO by immunohistochemistry for VCAM1 and DAPI. Insets show CA1 region of both hemispheres. (C) Quality control of Gpr37l1 KO by immunohistochemistry for GPR37L1 and DAPI. Insets show CA1 region of both hemispheres. (D) Heatmap showing the log2 fold change in synaptic metrics comparing LacZ-gRNA and VCAM1-gRNA hemispheres across hippocampal laminae. For each synaptic metric and lamina, 3 FOVs (sections) per brain from 4 brains were analyzed. Log2-transformed metrics were analyzed using a linear mixed-effects model with gRNA, hippocampal lamina and their interaction as fixed effects to test gene- and lamina-specific effects, and brain as a random intercept to account for inter-brain variability. Asterisks denote significant differences after false discovery rate (FDR) correction for each metric across hippocampal laminae (FDR < 0.05). (E) Heatmap showing the log2 fold change in synaptic metrics comparing LacZ-gRNA and GPR37L1-gRNA hemispheres. Same sampling, plotting and analyses as in (G). (F) Representative processed Airyscan images of VGLUT1 and PSD95 in CA1 SR from LacZ-gRNA- and VCAM1-gRNA-injected hemispheres, with quantification of local peak max and presynapse MFI normalized to the LacZ-gRNA hemisphere. Each small data point represents a single FOV; larger linked data points represent hemisphere means. Statistical significance was assessed using a linear mixed effects model as in (G), followed by Tukey’s post hoc tests and FDR corrected significance. (G) Representative processed Airyscan images of VGLUT1 and PSD95 in CA3 SR from LacZ-gRNA- and VCAM1-gRNA-injected hemispheres, with quantification of local peak max and presynapse MFI normalized to the LacZ-gRNA hemisphere. Same sampling, plotting and analyses as in (F). (H) Schematic of the workflow used to assess the effect <t>of</t> <t>VCAM1-Fc</t> protein treatment on hippocampal synapses in vitro . Created with BioRender.com. (I) Representative images of hippocampal neurons treated with recombinant protein from DIV8 to DIV14. Fc or VCAM1-Fc (2 µg/mL) was added at DIV8 and replenished at DIV11. Neurons were fixed at DIV14 and stained for VGLUT1 (cyan), PSD95 (magenta), and MAP2 (yellow). Insets show the merge followed by the isolated VGLUT1-PSD95 signal. (J) Quantification of excitatory synaptic puncta density along MAP2+ dendrites. VGLUT1+PSD95+ colocalized puncta, as well as VGLUT1+ and PSD95+ puncta separately, were measured per 100 μm dendrite and normalized to the Fc condition. Each FOV contained two neuronal somas for standardized sampling; 9-11 FOVs per condition were analyzed in N = 4 independent experiments. Each data point represents one experiment is color-shaded by experiment. A linear mixed-effects model on log2 transformed data was used to account for inter-experimental variability. Post-hoc pairwise comparisons were conducted using Tukey’s HSD test. Individual FOV data points are shown in .
    Recombinant Mouse Vcam1 Fc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+vcam/Recombinant+Mouse+VCAM-1%2FCD106+Fc+Chimera+Protein%2C+CF/bio_rxiv__64898__2026__03__17__712320-577-3-6
    Average 94 stars, based on 41 article reviews
    recombinant mouse vcam1 fc - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "A systematic cross-modal approach identifies astrocytic VCAM1 as a regulator of hippocampal synapse development"

    Article Title: A systematic cross-modal approach identifies astrocytic VCAM1 as a regulator of hippocampal synapse development

    Journal: bioRxiv

    doi: 10.64898/2026.03.17.712320

    (A) Schematic of the workflow used to assess the effect of constitutive CRISPR/Cas9-mediated KO of Vcam1 or Gpr37l1 on hippocampal synapse development. SO: stratum oriens, SR: stratum radiatum, SLM: stratum lacunosum-moleculare, SL: stratum lucidum, DG: dentate gyrus, ML: molecular layer. Created with BioRender.com. (B) Quality control of Vcam1 KO by immunohistochemistry for VCAM1 and DAPI. Insets show CA1 region of both hemispheres. (C) Quality control of Gpr37l1 KO by immunohistochemistry for GPR37L1 and DAPI. Insets show CA1 region of both hemispheres. (D) Heatmap showing the log2 fold change in synaptic metrics comparing LacZ-gRNA and VCAM1-gRNA hemispheres across hippocampal laminae. For each synaptic metric and lamina, 3 FOVs (sections) per brain from 4 brains were analyzed. Log2-transformed metrics were analyzed using a linear mixed-effects model with gRNA, hippocampal lamina and their interaction as fixed effects to test gene- and lamina-specific effects, and brain as a random intercept to account for inter-brain variability. Asterisks denote significant differences after false discovery rate (FDR) correction for each metric across hippocampal laminae (FDR < 0.05). (E) Heatmap showing the log2 fold change in synaptic metrics comparing LacZ-gRNA and GPR37L1-gRNA hemispheres. Same sampling, plotting and analyses as in (G). (F) Representative processed Airyscan images of VGLUT1 and PSD95 in CA1 SR from LacZ-gRNA- and VCAM1-gRNA-injected hemispheres, with quantification of local peak max and presynapse MFI normalized to the LacZ-gRNA hemisphere. Each small data point represents a single FOV; larger linked data points represent hemisphere means. Statistical significance was assessed using a linear mixed effects model as in (G), followed by Tukey’s post hoc tests and FDR corrected significance. (G) Representative processed Airyscan images of VGLUT1 and PSD95 in CA3 SR from LacZ-gRNA- and VCAM1-gRNA-injected hemispheres, with quantification of local peak max and presynapse MFI normalized to the LacZ-gRNA hemisphere. Same sampling, plotting and analyses as in (F). (H) Schematic of the workflow used to assess the effect of VCAM1-Fc protein treatment on hippocampal synapses in vitro . Created with BioRender.com. (I) Representative images of hippocampal neurons treated with recombinant protein from DIV8 to DIV14. Fc or VCAM1-Fc (2 µg/mL) was added at DIV8 and replenished at DIV11. Neurons were fixed at DIV14 and stained for VGLUT1 (cyan), PSD95 (magenta), and MAP2 (yellow). Insets show the merge followed by the isolated VGLUT1-PSD95 signal. (J) Quantification of excitatory synaptic puncta density along MAP2+ dendrites. VGLUT1+PSD95+ colocalized puncta, as well as VGLUT1+ and PSD95+ puncta separately, were measured per 100 μm dendrite and normalized to the Fc condition. Each FOV contained two neuronal somas for standardized sampling; 9-11 FOVs per condition were analyzed in N = 4 independent experiments. Each data point represents one experiment is color-shaded by experiment. A linear mixed-effects model on log2 transformed data was used to account for inter-experimental variability. Post-hoc pairwise comparisons were conducted using Tukey’s HSD test. Individual FOV data points are shown in .
    Figure Legend Snippet: (A) Schematic of the workflow used to assess the effect of constitutive CRISPR/Cas9-mediated KO of Vcam1 or Gpr37l1 on hippocampal synapse development. SO: stratum oriens, SR: stratum radiatum, SLM: stratum lacunosum-moleculare, SL: stratum lucidum, DG: dentate gyrus, ML: molecular layer. Created with BioRender.com. (B) Quality control of Vcam1 KO by immunohistochemistry for VCAM1 and DAPI. Insets show CA1 region of both hemispheres. (C) Quality control of Gpr37l1 KO by immunohistochemistry for GPR37L1 and DAPI. Insets show CA1 region of both hemispheres. (D) Heatmap showing the log2 fold change in synaptic metrics comparing LacZ-gRNA and VCAM1-gRNA hemispheres across hippocampal laminae. For each synaptic metric and lamina, 3 FOVs (sections) per brain from 4 brains were analyzed. Log2-transformed metrics were analyzed using a linear mixed-effects model with gRNA, hippocampal lamina and their interaction as fixed effects to test gene- and lamina-specific effects, and brain as a random intercept to account for inter-brain variability. Asterisks denote significant differences after false discovery rate (FDR) correction for each metric across hippocampal laminae (FDR < 0.05). (E) Heatmap showing the log2 fold change in synaptic metrics comparing LacZ-gRNA and GPR37L1-gRNA hemispheres. Same sampling, plotting and analyses as in (G). (F) Representative processed Airyscan images of VGLUT1 and PSD95 in CA1 SR from LacZ-gRNA- and VCAM1-gRNA-injected hemispheres, with quantification of local peak max and presynapse MFI normalized to the LacZ-gRNA hemisphere. Each small data point represents a single FOV; larger linked data points represent hemisphere means. Statistical significance was assessed using a linear mixed effects model as in (G), followed by Tukey’s post hoc tests and FDR corrected significance. (G) Representative processed Airyscan images of VGLUT1 and PSD95 in CA3 SR from LacZ-gRNA- and VCAM1-gRNA-injected hemispheres, with quantification of local peak max and presynapse MFI normalized to the LacZ-gRNA hemisphere. Same sampling, plotting and analyses as in (F). (H) Schematic of the workflow used to assess the effect of VCAM1-Fc protein treatment on hippocampal synapses in vitro . Created with BioRender.com. (I) Representative images of hippocampal neurons treated with recombinant protein from DIV8 to DIV14. Fc or VCAM1-Fc (2 µg/mL) was added at DIV8 and replenished at DIV11. Neurons were fixed at DIV14 and stained for VGLUT1 (cyan), PSD95 (magenta), and MAP2 (yellow). Insets show the merge followed by the isolated VGLUT1-PSD95 signal. (J) Quantification of excitatory synaptic puncta density along MAP2+ dendrites. VGLUT1+PSD95+ colocalized puncta, as well as VGLUT1+ and PSD95+ puncta separately, were measured per 100 μm dendrite and normalized to the Fc condition. Each FOV contained two neuronal somas for standardized sampling; 9-11 FOVs per condition were analyzed in N = 4 independent experiments. Each data point represents one experiment is color-shaded by experiment. A linear mixed-effects model on log2 transformed data was used to account for inter-experimental variability. Post-hoc pairwise comparisons were conducted using Tukey’s HSD test. Individual FOV data points are shown in .

    Techniques Used: CRISPR, Control, Immunohistochemistry, Transformation Assay, Sampling, Injection, In Vitro, Recombinant, Staining, Isolation

    (A) Western blot quality control of the recombinant proteins Fc and VCAM1-Fc used to treat hippocampal neurons, visualized using a total protein stain. (B) Representative images of hippocampal neurons treated with recombinant protein from DIV8 to DIV14. Fc or VCAM1–Fc (2 µg/mL) was added at DIV8 and replenished at DIV11. Neurons were fixed at DIV14 and stained for VGAT (cyan), GEPH (magenta), and MAP2 (yellow). Insets show the merge followed by the isolated VGAT-GEPH signal. (C) Quantification of inhibitory synaptic puncta density along MAP2+ dendrites. VGAT+GEPH+ colocalized puncta, as well as VGAT+ and GEPH+ puncta separately, were measured per 100 μm dendrite and normalized to Fc. Same sampling, plotting and analyses as in . (D) Plots from showing distribution of the analyzed FOVs. Each dot represents a single FOV and is color-shaded according to experiment. (E) Plots from (C) showing distribution of the analyzed FOVs. Each dot represents a single FOV and is color-shaded according to experiment. (F) Quantification of excitatory synaptic puncta size of VGLUT1+PSD95+ colocalized puncta, as well as VGLUT1+ and PSD95+ puncta separately. Each FOV contained two neuronal somas for standardized sampling; 9-11 FOVs per condition were analyzed in N = 4 independent experiments. Each data point represents one experiment (above panel), and one FOV (below panel), color-shaded by experiment. A linear mixed-effects model was used to account for multiple FOVs per experiment. (G) Quantification of inhibitory synaptic puncta size of VGAT+GEPH+ colocalized puncta, as well as VGAT+ and GEPH+ puncta separately. Same sampling, plotting and analyses as in (F).
    Figure Legend Snippet: (A) Western blot quality control of the recombinant proteins Fc and VCAM1-Fc used to treat hippocampal neurons, visualized using a total protein stain. (B) Representative images of hippocampal neurons treated with recombinant protein from DIV8 to DIV14. Fc or VCAM1–Fc (2 µg/mL) was added at DIV8 and replenished at DIV11. Neurons were fixed at DIV14 and stained for VGAT (cyan), GEPH (magenta), and MAP2 (yellow). Insets show the merge followed by the isolated VGAT-GEPH signal. (C) Quantification of inhibitory synaptic puncta density along MAP2+ dendrites. VGAT+GEPH+ colocalized puncta, as well as VGAT+ and GEPH+ puncta separately, were measured per 100 μm dendrite and normalized to Fc. Same sampling, plotting and analyses as in . (D) Plots from showing distribution of the analyzed FOVs. Each dot represents a single FOV and is color-shaded according to experiment. (E) Plots from (C) showing distribution of the analyzed FOVs. Each dot represents a single FOV and is color-shaded according to experiment. (F) Quantification of excitatory synaptic puncta size of VGLUT1+PSD95+ colocalized puncta, as well as VGLUT1+ and PSD95+ puncta separately. Each FOV contained two neuronal somas for standardized sampling; 9-11 FOVs per condition were analyzed in N = 4 independent experiments. Each data point represents one experiment (above panel), and one FOV (below panel), color-shaded by experiment. A linear mixed-effects model was used to account for multiple FOVs per experiment. (G) Quantification of inhibitory synaptic puncta size of VGAT+GEPH+ colocalized puncta, as well as VGAT+ and GEPH+ puncta separately. Same sampling, plotting and analyses as in (F).

    Techniques Used: Western Blot, Control, Recombinant, Staining, Isolation, Sampling

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    (A) Schematic of the workflow used to assess the effect of constitutive CRISPR/Cas9-mediated KO of <t>Vcam1</t> or Gpr37l1 on hippocampal synapse development. SO: stratum oriens, SR: stratum radiatum, SLM: stratum lacunosum-moleculare, SL: stratum lucidum, DG: dentate gyrus, ML: molecular layer. Created with BioRender.com. (B) Quality control of Vcam1 KO by immunohistochemistry for VCAM1 and DAPI. Insets show CA1 region of both hemispheres. (C) Quality control of Gpr37l1 KO by immunohistochemistry for GPR37L1 and DAPI. Insets show CA1 region of both hemispheres. (D) Heatmap showing the log2 fold change in synaptic metrics comparing LacZ-gRNA and VCAM1-gRNA hemispheres across hippocampal laminae. For each synaptic metric and lamina, 3 FOVs (sections) per brain from 4 brains were analyzed. Log2-transformed metrics were analyzed using a linear mixed-effects model with gRNA, hippocampal lamina and their interaction as fixed effects to test gene- and lamina-specific effects, and brain as a random intercept to account for inter-brain variability. Asterisks denote significant differences after false discovery rate (FDR) correction for each metric across hippocampal laminae (FDR < 0.05). (E) Heatmap showing the log2 fold change in synaptic metrics comparing LacZ-gRNA and GPR37L1-gRNA hemispheres. Same sampling, plotting and analyses as in (G). (F) Representative processed Airyscan images of VGLUT1 and PSD95 in CA1 SR from LacZ-gRNA- and VCAM1-gRNA-injected hemispheres, with quantification of local peak max and presynapse MFI normalized to the LacZ-gRNA hemisphere. Each small data point represents a single FOV; larger linked data points represent hemisphere means. Statistical significance was assessed using a linear mixed effects model as in (G), followed by Tukey’s post hoc tests and FDR corrected significance. (G) Representative processed Airyscan images of VGLUT1 and PSD95 in CA3 SR from LacZ-gRNA- and VCAM1-gRNA-injected hemispheres, with quantification of local peak max and presynapse MFI normalized to the LacZ-gRNA hemisphere. Same sampling, plotting and analyses as in (F). (H) Schematic of the workflow used to assess the effect <t>of</t> <t>VCAM1-Fc</t> protein treatment on hippocampal synapses in vitro . Created with BioRender.com. (I) Representative images of hippocampal neurons treated with recombinant protein from DIV8 to DIV14. Fc or VCAM1-Fc (2 µg/mL) was added at DIV8 and replenished at DIV11. Neurons were fixed at DIV14 and stained for VGLUT1 (cyan), PSD95 (magenta), and MAP2 (yellow). Insets show the merge followed by the isolated VGLUT1-PSD95 signal. (J) Quantification of excitatory synaptic puncta density along MAP2+ dendrites. VGLUT1+PSD95+ colocalized puncta, as well as VGLUT1+ and PSD95+ puncta separately, were measured per 100 μm dendrite and normalized to the Fc condition. Each FOV contained two neuronal somas for standardized sampling; 9-11 FOVs per condition were analyzed in N = 4 independent experiments. Each data point represents one experiment is color-shaded by experiment. A linear mixed-effects model on log2 transformed data was used to account for inter-experimental variability. Post-hoc pairwise comparisons were conducted using Tukey’s HSD test. Individual FOV data points are shown in .
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    (A) Schematic of the workflow used to assess the effect of constitutive CRISPR/Cas9-mediated KO of <t>Vcam1</t> or Gpr37l1 on hippocampal synapse development. SO: stratum oriens, SR: stratum radiatum, SLM: stratum lacunosum-moleculare, SL: stratum lucidum, DG: dentate gyrus, ML: molecular layer. Created with BioRender.com. (B) Quality control of Vcam1 KO by immunohistochemistry for VCAM1 and DAPI. Insets show CA1 region of both hemispheres. (C) Quality control of Gpr37l1 KO by immunohistochemistry for GPR37L1 and DAPI. Insets show CA1 region of both hemispheres. (D) Heatmap showing the log2 fold change in synaptic metrics comparing LacZ-gRNA and VCAM1-gRNA hemispheres across hippocampal laminae. For each synaptic metric and lamina, 3 FOVs (sections) per brain from 4 brains were analyzed. Log2-transformed metrics were analyzed using a linear mixed-effects model with gRNA, hippocampal lamina and their interaction as fixed effects to test gene- and lamina-specific effects, and brain as a random intercept to account for inter-brain variability. Asterisks denote significant differences after false discovery rate (FDR) correction for each metric across hippocampal laminae (FDR < 0.05). (E) Heatmap showing the log2 fold change in synaptic metrics comparing LacZ-gRNA and GPR37L1-gRNA hemispheres. Same sampling, plotting and analyses as in (G). (F) Representative processed Airyscan images of VGLUT1 and PSD95 in CA1 SR from LacZ-gRNA- and VCAM1-gRNA-injected hemispheres, with quantification of local peak max and presynapse MFI normalized to the LacZ-gRNA hemisphere. Each small data point represents a single FOV; larger linked data points represent hemisphere means. Statistical significance was assessed using a linear mixed effects model as in (G), followed by Tukey’s post hoc tests and FDR corrected significance. (G) Representative processed Airyscan images of VGLUT1 and PSD95 in CA3 SR from LacZ-gRNA- and VCAM1-gRNA-injected hemispheres, with quantification of local peak max and presynapse MFI normalized to the LacZ-gRNA hemisphere. Same sampling, plotting and analyses as in (F). (H) Schematic of the workflow used to assess the effect <t>of</t> <t>VCAM1-Fc</t> protein treatment on hippocampal synapses in vitro . Created with BioRender.com. (I) Representative images of hippocampal neurons treated with recombinant protein from DIV8 to DIV14. Fc or VCAM1-Fc (2 µg/mL) was added at DIV8 and replenished at DIV11. Neurons were fixed at DIV14 and stained for VGLUT1 (cyan), PSD95 (magenta), and MAP2 (yellow). Insets show the merge followed by the isolated VGLUT1-PSD95 signal. (J) Quantification of excitatory synaptic puncta density along MAP2+ dendrites. VGLUT1+PSD95+ colocalized puncta, as well as VGLUT1+ and PSD95+ puncta separately, were measured per 100 μm dendrite and normalized to the Fc condition. Each FOV contained two neuronal somas for standardized sampling; 9-11 FOVs per condition were analyzed in N = 4 independent experiments. Each data point represents one experiment is color-shaded by experiment. A linear mixed-effects model on log2 transformed data was used to account for inter-experimental variability. Post-hoc pairwise comparisons were conducted using Tukey’s HSD test. Individual FOV data points are shown in .
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    94
    R&D Systems recombinant mouse vcam 1
    (A) Schematic of the workflow used to assess the effect of constitutive CRISPR/Cas9-mediated KO of <t>Vcam1</t> or Gpr37l1 on hippocampal synapse development. SO: stratum oriens, SR: stratum radiatum, SLM: stratum lacunosum-moleculare, SL: stratum lucidum, DG: dentate gyrus, ML: molecular layer. Created with BioRender.com. (B) Quality control of Vcam1 KO by immunohistochemistry for VCAM1 and DAPI. Insets show CA1 region of both hemispheres. (C) Quality control of Gpr37l1 KO by immunohistochemistry for GPR37L1 and DAPI. Insets show CA1 region of both hemispheres. (D) Heatmap showing the log2 fold change in synaptic metrics comparing LacZ-gRNA and VCAM1-gRNA hemispheres across hippocampal laminae. For each synaptic metric and lamina, 3 FOVs (sections) per brain from 4 brains were analyzed. Log2-transformed metrics were analyzed using a linear mixed-effects model with gRNA, hippocampal lamina and their interaction as fixed effects to test gene- and lamina-specific effects, and brain as a random intercept to account for inter-brain variability. Asterisks denote significant differences after false discovery rate (FDR) correction for each metric across hippocampal laminae (FDR < 0.05). (E) Heatmap showing the log2 fold change in synaptic metrics comparing LacZ-gRNA and GPR37L1-gRNA hemispheres. Same sampling, plotting and analyses as in (G). (F) Representative processed Airyscan images of VGLUT1 and PSD95 in CA1 SR from LacZ-gRNA- and VCAM1-gRNA-injected hemispheres, with quantification of local peak max and presynapse MFI normalized to the LacZ-gRNA hemisphere. Each small data point represents a single FOV; larger linked data points represent hemisphere means. Statistical significance was assessed using a linear mixed effects model as in (G), followed by Tukey’s post hoc tests and FDR corrected significance. (G) Representative processed Airyscan images of VGLUT1 and PSD95 in CA3 SR from LacZ-gRNA- and VCAM1-gRNA-injected hemispheres, with quantification of local peak max and presynapse MFI normalized to the LacZ-gRNA hemisphere. Same sampling, plotting and analyses as in (F). (H) Schematic of the workflow used to assess the effect <t>of</t> <t>VCAM1-Fc</t> protein treatment on hippocampal synapses in vitro . Created with BioRender.com. (I) Representative images of hippocampal neurons treated with recombinant protein from DIV8 to DIV14. Fc or VCAM1-Fc (2 µg/mL) was added at DIV8 and replenished at DIV11. Neurons were fixed at DIV14 and stained for VGLUT1 (cyan), PSD95 (magenta), and MAP2 (yellow). Insets show the merge followed by the isolated VGLUT1-PSD95 signal. (J) Quantification of excitatory synaptic puncta density along MAP2+ dendrites. VGLUT1+PSD95+ colocalized puncta, as well as VGLUT1+ and PSD95+ puncta separately, were measured per 100 μm dendrite and normalized to the Fc condition. Each FOV contained two neuronal somas for standardized sampling; 9-11 FOVs per condition were analyzed in N = 4 independent experiments. Each data point represents one experiment is color-shaded by experiment. A linear mixed-effects model on log2 transformed data was used to account for inter-experimental variability. Post-hoc pairwise comparisons were conducted using Tukey’s HSD test. Individual FOV data points are shown in .
    Recombinant Mouse Vcam 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+vcam/Recombinant+Mouse+VCAM-1%2FCD106+Fc+Chimera+Protein%2C+CF/bio_rxiv__2025__10__03__680076-273-17-20
    Average 94 stars, based on 1 article reviews
    recombinant mouse vcam 1 - by Bioz Stars, 2026-09
    94/100 stars
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    rmvcam 1 fc chimera  (R&D Systems)
    94
    R&D Systems rmvcam 1 fc chimera
    (A) Schematic of the workflow used to assess the effect of constitutive CRISPR/Cas9-mediated KO of <t>Vcam1</t> or Gpr37l1 on hippocampal synapse development. SO: stratum oriens, SR: stratum radiatum, SLM: stratum lacunosum-moleculare, SL: stratum lucidum, DG: dentate gyrus, ML: molecular layer. Created with BioRender.com. (B) Quality control of Vcam1 KO by immunohistochemistry for VCAM1 and DAPI. Insets show CA1 region of both hemispheres. (C) Quality control of Gpr37l1 KO by immunohistochemistry for GPR37L1 and DAPI. Insets show CA1 region of both hemispheres. (D) Heatmap showing the log2 fold change in synaptic metrics comparing LacZ-gRNA and VCAM1-gRNA hemispheres across hippocampal laminae. For each synaptic metric and lamina, 3 FOVs (sections) per brain from 4 brains were analyzed. Log2-transformed metrics were analyzed using a linear mixed-effects model with gRNA, hippocampal lamina and their interaction as fixed effects to test gene- and lamina-specific effects, and brain as a random intercept to account for inter-brain variability. Asterisks denote significant differences after false discovery rate (FDR) correction for each metric across hippocampal laminae (FDR < 0.05). (E) Heatmap showing the log2 fold change in synaptic metrics comparing LacZ-gRNA and GPR37L1-gRNA hemispheres. Same sampling, plotting and analyses as in (G). (F) Representative processed Airyscan images of VGLUT1 and PSD95 in CA1 SR from LacZ-gRNA- and VCAM1-gRNA-injected hemispheres, with quantification of local peak max and presynapse MFI normalized to the LacZ-gRNA hemisphere. Each small data point represents a single FOV; larger linked data points represent hemisphere means. Statistical significance was assessed using a linear mixed effects model as in (G), followed by Tukey’s post hoc tests and FDR corrected significance. (G) Representative processed Airyscan images of VGLUT1 and PSD95 in CA3 SR from LacZ-gRNA- and VCAM1-gRNA-injected hemispheres, with quantification of local peak max and presynapse MFI normalized to the LacZ-gRNA hemisphere. Same sampling, plotting and analyses as in (F). (H) Schematic of the workflow used to assess the effect <t>of</t> <t>VCAM1-Fc</t> protein treatment on hippocampal synapses in vitro . Created with BioRender.com. (I) Representative images of hippocampal neurons treated with recombinant protein from DIV8 to DIV14. Fc or VCAM1-Fc (2 µg/mL) was added at DIV8 and replenished at DIV11. Neurons were fixed at DIV14 and stained for VGLUT1 (cyan), PSD95 (magenta), and MAP2 (yellow). Insets show the merge followed by the isolated VGLUT1-PSD95 signal. (J) Quantification of excitatory synaptic puncta density along MAP2+ dendrites. VGLUT1+PSD95+ colocalized puncta, as well as VGLUT1+ and PSD95+ puncta separately, were measured per 100 μm dendrite and normalized to the Fc condition. Each FOV contained two neuronal somas for standardized sampling; 9-11 FOVs per condition were analyzed in N = 4 independent experiments. Each data point represents one experiment is color-shaded by experiment. A linear mixed-effects model on log2 transformed data was used to account for inter-experimental variability. Post-hoc pairwise comparisons were conducted using Tukey’s HSD test. Individual FOV data points are shown in .
    Rmvcam 1 Fc Chimera, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+vcam/Recombinant+Mouse+VCAM-1%2FCD106+Fc+Chimera+Protein%2C+CF/pm40420242-78-30-35
    Average 94 stars, based on 1 article reviews
    rmvcam 1 fc chimera - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    rmvcam 1  (R&D Systems)
    94
    R&D Systems rmvcam 1
    (A) Schematic of the workflow used to assess the effect of constitutive CRISPR/Cas9-mediated KO of <t>Vcam1</t> or Gpr37l1 on hippocampal synapse development. SO: stratum oriens, SR: stratum radiatum, SLM: stratum lacunosum-moleculare, SL: stratum lucidum, DG: dentate gyrus, ML: molecular layer. Created with BioRender.com. (B) Quality control of Vcam1 KO by immunohistochemistry for VCAM1 and DAPI. Insets show CA1 region of both hemispheres. (C) Quality control of Gpr37l1 KO by immunohistochemistry for GPR37L1 and DAPI. Insets show CA1 region of both hemispheres. (D) Heatmap showing the log2 fold change in synaptic metrics comparing LacZ-gRNA and VCAM1-gRNA hemispheres across hippocampal laminae. For each synaptic metric and lamina, 3 FOVs (sections) per brain from 4 brains were analyzed. Log2-transformed metrics were analyzed using a linear mixed-effects model with gRNA, hippocampal lamina and their interaction as fixed effects to test gene- and lamina-specific effects, and brain as a random intercept to account for inter-brain variability. Asterisks denote significant differences after false discovery rate (FDR) correction for each metric across hippocampal laminae (FDR < 0.05). (E) Heatmap showing the log2 fold change in synaptic metrics comparing LacZ-gRNA and GPR37L1-gRNA hemispheres. Same sampling, plotting and analyses as in (G). (F) Representative processed Airyscan images of VGLUT1 and PSD95 in CA1 SR from LacZ-gRNA- and VCAM1-gRNA-injected hemispheres, with quantification of local peak max and presynapse MFI normalized to the LacZ-gRNA hemisphere. Each small data point represents a single FOV; larger linked data points represent hemisphere means. Statistical significance was assessed using a linear mixed effects model as in (G), followed by Tukey’s post hoc tests and FDR corrected significance. (G) Representative processed Airyscan images of VGLUT1 and PSD95 in CA3 SR from LacZ-gRNA- and VCAM1-gRNA-injected hemispheres, with quantification of local peak max and presynapse MFI normalized to the LacZ-gRNA hemisphere. Same sampling, plotting and analyses as in (F). (H) Schematic of the workflow used to assess the effect <t>of</t> <t>VCAM1-Fc</t> protein treatment on hippocampal synapses in vitro . Created with BioRender.com. (I) Representative images of hippocampal neurons treated with recombinant protein from DIV8 to DIV14. Fc or VCAM1-Fc (2 µg/mL) was added at DIV8 and replenished at DIV11. Neurons were fixed at DIV14 and stained for VGLUT1 (cyan), PSD95 (magenta), and MAP2 (yellow). Insets show the merge followed by the isolated VGLUT1-PSD95 signal. (J) Quantification of excitatory synaptic puncta density along MAP2+ dendrites. VGLUT1+PSD95+ colocalized puncta, as well as VGLUT1+ and PSD95+ puncta separately, were measured per 100 μm dendrite and normalized to the Fc condition. Each FOV contained two neuronal somas for standardized sampling; 9-11 FOVs per condition were analyzed in N = 4 independent experiments. Each data point represents one experiment is color-shaded by experiment. A linear mixed-effects model on log2 transformed data was used to account for inter-experimental variability. Post-hoc pairwise comparisons were conducted using Tukey’s HSD test. Individual FOV data points are shown in .
    Rmvcam 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+vcam/Recombinant+Mouse+VCAM-1%2FCD106+Fc+Chimera+Protein%2C+CF/pm40420242-83-33-37
    Average 94 stars, based on 1 article reviews
    rmvcam 1 - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    recombinant mouse vcam 1 fc chimera protein  (R&D Systems)
    94
    R&D Systems recombinant mouse vcam 1 fc chimera protein
    (A) Schematic of the workflow used to assess the effect of constitutive CRISPR/Cas9-mediated KO of <t>Vcam1</t> or Gpr37l1 on hippocampal synapse development. SO: stratum oriens, SR: stratum radiatum, SLM: stratum lacunosum-moleculare, SL: stratum lucidum, DG: dentate gyrus, ML: molecular layer. Created with BioRender.com. (B) Quality control of Vcam1 KO by immunohistochemistry for VCAM1 and DAPI. Insets show CA1 region of both hemispheres. (C) Quality control of Gpr37l1 KO by immunohistochemistry for GPR37L1 and DAPI. Insets show CA1 region of both hemispheres. (D) Heatmap showing the log2 fold change in synaptic metrics comparing LacZ-gRNA and VCAM1-gRNA hemispheres across hippocampal laminae. For each synaptic metric and lamina, 3 FOVs (sections) per brain from 4 brains were analyzed. Log2-transformed metrics were analyzed using a linear mixed-effects model with gRNA, hippocampal lamina and their interaction as fixed effects to test gene- and lamina-specific effects, and brain as a random intercept to account for inter-brain variability. Asterisks denote significant differences after false discovery rate (FDR) correction for each metric across hippocampal laminae (FDR < 0.05). (E) Heatmap showing the log2 fold change in synaptic metrics comparing LacZ-gRNA and GPR37L1-gRNA hemispheres. Same sampling, plotting and analyses as in (G). (F) Representative processed Airyscan images of VGLUT1 and PSD95 in CA1 SR from LacZ-gRNA- and VCAM1-gRNA-injected hemispheres, with quantification of local peak max and presynapse MFI normalized to the LacZ-gRNA hemisphere. Each small data point represents a single FOV; larger linked data points represent hemisphere means. Statistical significance was assessed using a linear mixed effects model as in (G), followed by Tukey’s post hoc tests and FDR corrected significance. (G) Representative processed Airyscan images of VGLUT1 and PSD95 in CA3 SR from LacZ-gRNA- and VCAM1-gRNA-injected hemispheres, with quantification of local peak max and presynapse MFI normalized to the LacZ-gRNA hemisphere. Same sampling, plotting and analyses as in (F). (H) Schematic of the workflow used to assess the effect <t>of</t> <t>VCAM1-Fc</t> protein treatment on hippocampal synapses in vitro . Created with BioRender.com. (I) Representative images of hippocampal neurons treated with recombinant protein from DIV8 to DIV14. Fc or VCAM1-Fc (2 µg/mL) was added at DIV8 and replenished at DIV11. Neurons were fixed at DIV14 and stained for VGLUT1 (cyan), PSD95 (magenta), and MAP2 (yellow). Insets show the merge followed by the isolated VGLUT1-PSD95 signal. (J) Quantification of excitatory synaptic puncta density along MAP2+ dendrites. VGLUT1+PSD95+ colocalized puncta, as well as VGLUT1+ and PSD95+ puncta separately, were measured per 100 μm dendrite and normalized to the Fc condition. Each FOV contained two neuronal somas for standardized sampling; 9-11 FOVs per condition were analyzed in N = 4 independent experiments. Each data point represents one experiment is color-shaded by experiment. A linear mixed-effects model on log2 transformed data was used to account for inter-experimental variability. Post-hoc pairwise comparisons were conducted using Tukey’s HSD test. Individual FOV data points are shown in .
    Recombinant Mouse Vcam 1 Fc Chimera Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+vcam/Recombinant+Mouse+VCAM-1%2FCD106+Fc+Chimera+Protein%2C+CF/bio_rxiv__2025__02__19__638920-217-15-22
    Average 94 stars, based on 1 article reviews
    recombinant mouse vcam 1 fc chimera protein - by Bioz Stars, 2026-09
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    		  Buy from Supplier

    Image Search Results


    (A) Schematic of the workflow used to assess the effect of constitutive CRISPR/Cas9-mediated KO of Vcam1 or Gpr37l1 on hippocampal synapse development. SO: stratum oriens, SR: stratum radiatum, SLM: stratum lacunosum-moleculare, SL: stratum lucidum, DG: dentate gyrus, ML: molecular layer. Created with BioRender.com. (B) Quality control of Vcam1 KO by immunohistochemistry for VCAM1 and DAPI. Insets show CA1 region of both hemispheres. (C) Quality control of Gpr37l1 KO by immunohistochemistry for GPR37L1 and DAPI. Insets show CA1 region of both hemispheres. (D) Heatmap showing the log2 fold change in synaptic metrics comparing LacZ-gRNA and VCAM1-gRNA hemispheres across hippocampal laminae. For each synaptic metric and lamina, 3 FOVs (sections) per brain from 4 brains were analyzed. Log2-transformed metrics were analyzed using a linear mixed-effects model with gRNA, hippocampal lamina and their interaction as fixed effects to test gene- and lamina-specific effects, and brain as a random intercept to account for inter-brain variability. Asterisks denote significant differences after false discovery rate (FDR) correction for each metric across hippocampal laminae (FDR < 0.05). (E) Heatmap showing the log2 fold change in synaptic metrics comparing LacZ-gRNA and GPR37L1-gRNA hemispheres. Same sampling, plotting and analyses as in (G). (F) Representative processed Airyscan images of VGLUT1 and PSD95 in CA1 SR from LacZ-gRNA- and VCAM1-gRNA-injected hemispheres, with quantification of local peak max and presynapse MFI normalized to the LacZ-gRNA hemisphere. Each small data point represents a single FOV; larger linked data points represent hemisphere means. Statistical significance was assessed using a linear mixed effects model as in (G), followed by Tukey’s post hoc tests and FDR corrected significance. (G) Representative processed Airyscan images of VGLUT1 and PSD95 in CA3 SR from LacZ-gRNA- and VCAM1-gRNA-injected hemispheres, with quantification of local peak max and presynapse MFI normalized to the LacZ-gRNA hemisphere. Same sampling, plotting and analyses as in (F). (H) Schematic of the workflow used to assess the effect of VCAM1-Fc protein treatment on hippocampal synapses in vitro . Created with BioRender.com. (I) Representative images of hippocampal neurons treated with recombinant protein from DIV8 to DIV14. Fc or VCAM1-Fc (2 µg/mL) was added at DIV8 and replenished at DIV11. Neurons were fixed at DIV14 and stained for VGLUT1 (cyan), PSD95 (magenta), and MAP2 (yellow). Insets show the merge followed by the isolated VGLUT1-PSD95 signal. (J) Quantification of excitatory synaptic puncta density along MAP2+ dendrites. VGLUT1+PSD95+ colocalized puncta, as well as VGLUT1+ and PSD95+ puncta separately, were measured per 100 μm dendrite and normalized to the Fc condition. Each FOV contained two neuronal somas for standardized sampling; 9-11 FOVs per condition were analyzed in N = 4 independent experiments. Each data point represents one experiment is color-shaded by experiment. A linear mixed-effects model on log2 transformed data was used to account for inter-experimental variability. Post-hoc pairwise comparisons were conducted using Tukey’s HSD test. Individual FOV data points are shown in .

    Journal: bioRxiv

    Article Title: A systematic cross-modal approach identifies astrocytic VCAM1 as a regulator of hippocampal synapse development

    doi: 10.64898/2026.03.17.712320

    Figure Lengend Snippet: (A) Schematic of the workflow used to assess the effect of constitutive CRISPR/Cas9-mediated KO of Vcam1 or Gpr37l1 on hippocampal synapse development. SO: stratum oriens, SR: stratum radiatum, SLM: stratum lacunosum-moleculare, SL: stratum lucidum, DG: dentate gyrus, ML: molecular layer. Created with BioRender.com. (B) Quality control of Vcam1 KO by immunohistochemistry for VCAM1 and DAPI. Insets show CA1 region of both hemispheres. (C) Quality control of Gpr37l1 KO by immunohistochemistry for GPR37L1 and DAPI. Insets show CA1 region of both hemispheres. (D) Heatmap showing the log2 fold change in synaptic metrics comparing LacZ-gRNA and VCAM1-gRNA hemispheres across hippocampal laminae. For each synaptic metric and lamina, 3 FOVs (sections) per brain from 4 brains were analyzed. Log2-transformed metrics were analyzed using a linear mixed-effects model with gRNA, hippocampal lamina and their interaction as fixed effects to test gene- and lamina-specific effects, and brain as a random intercept to account for inter-brain variability. Asterisks denote significant differences after false discovery rate (FDR) correction for each metric across hippocampal laminae (FDR < 0.05). (E) Heatmap showing the log2 fold change in synaptic metrics comparing LacZ-gRNA and GPR37L1-gRNA hemispheres. Same sampling, plotting and analyses as in (G). (F) Representative processed Airyscan images of VGLUT1 and PSD95 in CA1 SR from LacZ-gRNA- and VCAM1-gRNA-injected hemispheres, with quantification of local peak max and presynapse MFI normalized to the LacZ-gRNA hemisphere. Each small data point represents a single FOV; larger linked data points represent hemisphere means. Statistical significance was assessed using a linear mixed effects model as in (G), followed by Tukey’s post hoc tests and FDR corrected significance. (G) Representative processed Airyscan images of VGLUT1 and PSD95 in CA3 SR from LacZ-gRNA- and VCAM1-gRNA-injected hemispheres, with quantification of local peak max and presynapse MFI normalized to the LacZ-gRNA hemisphere. Same sampling, plotting and analyses as in (F). (H) Schematic of the workflow used to assess the effect of VCAM1-Fc protein treatment on hippocampal synapses in vitro . Created with BioRender.com. (I) Representative images of hippocampal neurons treated with recombinant protein from DIV8 to DIV14. Fc or VCAM1-Fc (2 µg/mL) was added at DIV8 and replenished at DIV11. Neurons were fixed at DIV14 and stained for VGLUT1 (cyan), PSD95 (magenta), and MAP2 (yellow). Insets show the merge followed by the isolated VGLUT1-PSD95 signal. (J) Quantification of excitatory synaptic puncta density along MAP2+ dendrites. VGLUT1+PSD95+ colocalized puncta, as well as VGLUT1+ and PSD95+ puncta separately, were measured per 100 μm dendrite and normalized to the Fc condition. Each FOV contained two neuronal somas for standardized sampling; 9-11 FOVs per condition were analyzed in N = 4 independent experiments. Each data point represents one experiment is color-shaded by experiment. A linear mixed-effects model on log2 transformed data was used to account for inter-experimental variability. Post-hoc pairwise comparisons were conducted using Tukey’s HSD test. Individual FOV data points are shown in .

    Article Snippet: Upon arrival, lyophilized recombinant mouse VCAM1-Fc (R&D Systems, 643-VM) was briefly centrifuged and reconstituted in sterile Dulbecco’s phosphate-buffered saline (DPBS).

    Techniques: CRISPR, Control, Immunohistochemistry, Transformation Assay, Sampling, Injection, In Vitro, Recombinant, Staining, Isolation

    (A) Western blot quality control of the recombinant proteins Fc and VCAM1-Fc used to treat hippocampal neurons, visualized using a total protein stain. (B) Representative images of hippocampal neurons treated with recombinant protein from DIV8 to DIV14. Fc or VCAM1–Fc (2 µg/mL) was added at DIV8 and replenished at DIV11. Neurons were fixed at DIV14 and stained for VGAT (cyan), GEPH (magenta), and MAP2 (yellow). Insets show the merge followed by the isolated VGAT-GEPH signal. (C) Quantification of inhibitory synaptic puncta density along MAP2+ dendrites. VGAT+GEPH+ colocalized puncta, as well as VGAT+ and GEPH+ puncta separately, were measured per 100 μm dendrite and normalized to Fc. Same sampling, plotting and analyses as in . (D) Plots from showing distribution of the analyzed FOVs. Each dot represents a single FOV and is color-shaded according to experiment. (E) Plots from (C) showing distribution of the analyzed FOVs. Each dot represents a single FOV and is color-shaded according to experiment. (F) Quantification of excitatory synaptic puncta size of VGLUT1+PSD95+ colocalized puncta, as well as VGLUT1+ and PSD95+ puncta separately. Each FOV contained two neuronal somas for standardized sampling; 9-11 FOVs per condition were analyzed in N = 4 independent experiments. Each data point represents one experiment (above panel), and one FOV (below panel), color-shaded by experiment. A linear mixed-effects model was used to account for multiple FOVs per experiment. (G) Quantification of inhibitory synaptic puncta size of VGAT+GEPH+ colocalized puncta, as well as VGAT+ and GEPH+ puncta separately. Same sampling, plotting and analyses as in (F).

    Journal: bioRxiv

    Article Title: A systematic cross-modal approach identifies astrocytic VCAM1 as a regulator of hippocampal synapse development

    doi: 10.64898/2026.03.17.712320

    Figure Lengend Snippet: (A) Western blot quality control of the recombinant proteins Fc and VCAM1-Fc used to treat hippocampal neurons, visualized using a total protein stain. (B) Representative images of hippocampal neurons treated with recombinant protein from DIV8 to DIV14. Fc or VCAM1–Fc (2 µg/mL) was added at DIV8 and replenished at DIV11. Neurons were fixed at DIV14 and stained for VGAT (cyan), GEPH (magenta), and MAP2 (yellow). Insets show the merge followed by the isolated VGAT-GEPH signal. (C) Quantification of inhibitory synaptic puncta density along MAP2+ dendrites. VGAT+GEPH+ colocalized puncta, as well as VGAT+ and GEPH+ puncta separately, were measured per 100 μm dendrite and normalized to Fc. Same sampling, plotting and analyses as in . (D) Plots from showing distribution of the analyzed FOVs. Each dot represents a single FOV and is color-shaded according to experiment. (E) Plots from (C) showing distribution of the analyzed FOVs. Each dot represents a single FOV and is color-shaded according to experiment. (F) Quantification of excitatory synaptic puncta size of VGLUT1+PSD95+ colocalized puncta, as well as VGLUT1+ and PSD95+ puncta separately. Each FOV contained two neuronal somas for standardized sampling; 9-11 FOVs per condition were analyzed in N = 4 independent experiments. Each data point represents one experiment (above panel), and one FOV (below panel), color-shaded by experiment. A linear mixed-effects model was used to account for multiple FOVs per experiment. (G) Quantification of inhibitory synaptic puncta size of VGAT+GEPH+ colocalized puncta, as well as VGAT+ and GEPH+ puncta separately. Same sampling, plotting and analyses as in (F).

    Article Snippet: Upon arrival, lyophilized recombinant mouse VCAM1-Fc (R&D Systems, 643-VM) was briefly centrifuged and reconstituted in sterile Dulbecco’s phosphate-buffered saline (DPBS).

    Techniques: Western Blot, Control, Recombinant, Staining, Isolation, Sampling